n-Butanol production from sucrose and sugarcane juice by engineered Clostridium tyrobutyricum overexpressing sucrose catabolism genes and adhE2
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چکیده
منابع مشابه
Rebalancing Redox to Improve Biobutanol Production by Clostridium tyrobutyricum
Biobutanol is a sustainable green biofuel that can substitute for gasoline. Carbon flux has been redistributed in Clostridium tyrobutyricum via metabolic cell engineering to produce biobutanol. However, the lack of reducing power hampered the further improvement of butanol production. The objective of this study was to improve butanol production by rebalancing redox. Firstly, a metabolically-en...
متن کاملSucrose catabolism in Clostridium pasteurianum and its relation to N2 fixation.
The growth constant and Y (sucrose) (grams of cells per mole of sucrose) for NH(3)-grown cultures of Clostridium pasteurianum were 1.7 times those of N(2)-grown cultures, whereas the rate of sucrose utilized per gram of cells per hour was similar for both conditions. The Y (sucrose) of chemostat cultures grown on limiting NH(3) under argon at generation times equal to those of N(2)-fixing cultu...
متن کاملCo-culturing a novel Bacillus strain with Clostridium tyrobutyricum ATCC 25755 to produce butyric acid from sucrose
BACKGROUND Currently, the most promising microorganism used for the bio-production of butyric acid is Clostridium tyrobutyricum ATCC 25755T; however, it is unable to use sucrose as a sole carbon source. Consequently, a newly isolated strain, Bacillus sp. SGP1, that was found to produce a levansucrase enzyme, which hydrolyzes sucrose into fructose and glucose, was used in a co-culture with this ...
متن کاملIncreased Cellulose Production from Sucrose by Acetobacter after Introducing the Sucrose Phosphorylase Gene.
A sucrose phosphorylase (SPase) gene derived from Leuconostoc mesenteroides was introduced into a cellulose-producing Acetobacter strain and expressed under the lac promoter. The activity of the SPase was detected in extracts of the transformed cells and cellulose production from sucrose by the cells was found to have increased, which strongly suggests that the increase was the result of the ne...
متن کاملThe mechanism of polysaccharide production from sucrose.
The recent preparation of levansucrase, like that of dextransucrase and phosphorylase, brings a new and powerful tool to bear on the study of macromolecular biosynthesis (Hestrin, Avineri-Shapiro & Aschner, 1943; Hestrin & Avineri-Shapiro, 1943; Hehre & Sugg, 1942; Stacey, 1942, 1943; cf. also Cori, 1939; Hanes, 1940; Behrens & Bergmann, 1939). Recent work on polysaccharide production in vitro ...
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ژورنال
عنوان ژورنال: Bioresource Technology
سال: 2017
ISSN: 0960-8524
DOI: 10.1016/j.biortech.2017.02.079